6IX6
Crystal structure of the complex of peptidyl-tRNA hydrolase with N-propanol at 1.43 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-08 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96600 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.996, 66.474, 76.387 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.190 - 1.430 |
| R-factor | 0.15634 |
| Rwork | 0.154 |
| R-free | 0.19464 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y9a |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.828 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.190 | 1.550 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmeas | 0.075 | |
| Number of reflections | 32796 | |
| <I/σ(I)> | 9.11 | |
| Completeness [%] | 93.7 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 12% PEG 1500, 0.1M HEPES PH 7.5 |
