6IX1
Structure of 2S albumin seed protein from Dolichos
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.88560 |
Spacegroup name | P 31 |
Unit cell lengths | 65.158, 65.158, 118.167 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.410 - 1.280 |
R-factor | 0.13262 |
Rwork | 0.128 |
R-free | 0.16980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oyo |
RMSD bond length | 0.020 |
RMSD bond angle | 2.169 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (v1.8.2-1309) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.410 | 1.294 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.054 | 0.432 |
Rmeas | 0.067 | |
Rpim | 0.039 | 0.341 |
Number of reflections | 140457 | 13099 |
<I/σ(I)> | 15.6 | |
Completeness [%] | 97.0 | |
Redundancy | 2.8 | |
CC(1/2) | 0.986 | 0.866 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | Sodium acetate trihydrate, Ammonium sulphate |