6IWP

Structural insight into probable lipid transfer mechanism of non-specific lipid transfer protein via intermediate structures in Solanum melongena

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-11-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	I 41
Unit cell lengths	38.561, 38.561, 91.718
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.270 - 2.400
R-factor	0.1946
Rwork	0.191
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iwm
RMSD bond length	0.003
RMSD bond angle	0.618
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2747: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.118	0.572
Number of reflections	2517	197
<I/σ(I)>	15.16	2.56
Completeness [%]	94.6	75.4
Redundancy	5.2	4.1
CC(1/2)	0.925	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295.15	0.1M HEPES pH 7.5, 2M Ammonium sulfate