6IWP
Structural insight into probable lipid transfer mechanism of non-specific lipid transfer protein via intermediate structures in Solanum melongena
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-11-10 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | I 41 |
Unit cell lengths | 38.561, 38.561, 91.718 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.270 - 2.400 |
R-factor | 0.1946 |
Rwork | 0.191 |
R-free | 0.24170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iwm |
RMSD bond length | 0.003 |
RMSD bond angle | 0.618 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2747: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.118 | 0.572 |
Number of reflections | 2517 | 197 |
<I/σ(I)> | 15.16 | 2.56 |
Completeness [%] | 94.6 | 75.4 |
Redundancy | 5.2 | 4.1 |
CC(1/2) | 0.925 | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295.15 | 0.1M HEPES pH 7.5, 2M Ammonium sulfate |