6IWM
Structural insight into probable lipid transfer mechanism of non-specific lipid transfer protein via intermediate structures in Solanum melongena
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-02-24 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 61 |
Unit cell lengths | 87.036, 87.036, 50.342 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.130 - 1.980 |
R-factor | 0.1874 |
Rwork | 0.186 |
R-free | 0.21720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tvi |
RMSD bond angle | 0.930 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2747: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.010 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.064 | 0.639 |
Number of reflections | 15253 | 1507 |
<I/σ(I)> | 38.4 | 2.42 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.4 | 7.6 |
CC(1/2) | 0.955 | 0.748 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295.15 | 0.1M HEPES pH 7.0, 2M Ammonium sulfate |