6IWK

The Structure of Maltooligosaccharide-forming Amylase from Pseudomonas saccharophila STB07

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97892
Spacegroup name	P 21 21 21
Unit cell lengths	46.193, 64.768, 168.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.072 - 1.501
R-factor	0.198
Rwork	0.198
R-free	0.20710
RMSD bond length	0.005
RMSD bond angle	0.821
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.072	10.000
High resolution limit [Å]	1.501	2.000
Number of reflections	81383
<I/σ(I)>	1.37
Completeness [%]	99.4
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M ammonium sulfate, 20 mM Tris-HCl