6IWE

Crystal structure of fructosyl peptide oxidase thermally stable mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	80.251, 80.251, 130.347
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.540 - 1.939
R-factor	0.21
Rwork	0.208
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.949
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.520	2.000
High resolution limit [Å]	1.970	1.970
Number of reflections	36666
<I/σ(I)>	16
Completeness [%]	100.0
Redundancy	5.06

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M potassium phosphate dibasic, 20%(w/v) polyethylene glycol 3350