6IW6

Crystal structure of the Lin28-interacting module of human TUT4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	I 21 21 21
Unit cell lengths	113.270, 127.830, 168.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.963 - 2.402
R-factor	0.2113
Rwork	0.210
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w0n
RMSD bond length	0.010
RMSD bond angle	0.998
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12-2829_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.963	19.963	2.460
High resolution limit [Å]	2.400	10.740	2.400
Rmerge	0.190	0.033	2.372
Rmeas	0.194	0.034	2.419
Total number of observations	1288392
Number of reflections	48018	600	3482
<I/σ(I)>	18.11	75.89	1.69
Completeness [%]	99.9	98.2	99.3
Redundancy	26.831	21.363	26.144
CC(1/2)	0.999	1.000	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	100 mM Hepes-NaOH, pH 7.0, 20% (w/v) PEG 3350, 3% MPD, 200 mM Ammonium Citrate and 3% (w/v) 1,5-Diaminopentane dihydrochloride