6IW3
High resolution structure of Dvl2-DIX Y27W/C80S mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-18 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9000 |
Spacegroup name | P 61 |
Unit cell lengths | 44.470, 44.470, 87.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.500 - 1.640 |
R-factor | 0.2026 |
Rwork | 0.200 |
R-free | 0.22920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wip |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.133 | 2.300 |
Rmeas | 0.136 | 2.370 |
Number of reflections | 12127 | 873 |
<I/σ(I)> | 20.3 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.7 | 13.9 |
CC(1/2) | 0.999 | 0.511 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 20% (w/v) PEG 6000, Tris-HCl pH 8.0, 200 mM lithium chloride |