6IW1

Crystal structure of YFV-17D sE in postfusion state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	C 1 2 1
Unit cell lengths	140.558, 88.275, 128.953
Unit cell angles	90.00, 107.06, 90.00

Refinement procedure

Resolution	39.955 - 3.100
R-factor	0.217
Rwork	0.215
R-free	0.25390
RMSD bond length	0.006
RMSD bond angle	1.151
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	39.900
High resolution limit [Å]	3.100	3.100
Number of reflections	26089
<I/σ(I)>	7.8
Completeness [%]	99.4
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M HEPES pH 7.0, 30% v/v Jeffamine, M-600, pH 7.0