6IW0

Crystal structure of 5A ScFv in complex with YFV-17D sE in postfusion state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	H 3 2
Unit cell lengths	103.991, 103.991, 368.675
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.464 - 3.600
R-factor	0.2792
Rwork	0.277
R-free	0.33590
RMSD bond length	0.004
RMSD bond angle	0.881
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.730
High resolution limit [Å]	3.600	3.600
Number of reflections	8668
<I/σ(I)>	7.7
Completeness [%]	99.8
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	0.1 M Sodium malonate pH 4.0, 12% w/v Polyethylene glycol 3350