6IVZ

Crystal structure of 5A ScFv complexed with YFV-China sE in postfusion state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	H 3 2
Unit cell lengths	102.915, 102.915, 364.874
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.391 - 2.400
R-factor	0.2123
Rwork	0.210
R-free	0.25640
RMSD bond length	0.005
RMSD bond angle	0.850
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.400	2.400
Number of reflections	25919
<I/σ(I)>	22.8
Completeness [%]	99.9
Redundancy	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	0.1 M Sodium malonate pH 4.0, 12% w/v Polyethylene glycol 3350