6IVW

Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae with a mutation D269A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	113.966, 161.770, 162.371
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.186 - 3.720
R-factor	0.2931
Rwork	0.289
R-free	0.35150
RMSD bond length	0.004
RMSD bond angle	0.832
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	162.370	3.980
High resolution limit [Å]	3.720	3.720
Number of reflections	26197
<I/σ(I)>	5.5
Completeness [%]	81.3
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M zinc acetate, 6% v/v ethylene glycol, 0.1M sodium cacodylate, pH 6.0, 9% w/v PEG 8000