6IVJ
Crystal structure of a membrane protein G18A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 114.119, 160.027, 161.784 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.760 - 2.770 |
R-factor | 0.2168 |
Rwork | 0.215 |
R-free | 0.24434 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.614 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 161.780 | 2.820 |
High resolution limit [Å] | 2.770 | 2.770 |
Rmerge | 0.010 | 2.310 |
Rpim | 0.004 | 1.016 |
Number of reflections | 75940 | 4260 |
<I/σ(I)> | 15.5 | 0.8 |
Completeness [%] | 99.5 | 95.5 |
Redundancy | 6.7 | 5.9 |
CC(1/2) | 0.333 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.05 M zinc acetate, 6% v/v ethylene glycol, 0.1 M sodium cacodylate, pH 6.0, 6.6 % w/v PEG 8000 |