6IV9
the Cas13d binary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.224, 136.481, 147.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.110 - 1.860 |
| R-factor | 0.1712 |
| Rwork | 0.170 |
| R-free | 0.19560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6iv8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.735 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.110 | 1.960 |
| High resolution limit [Å] | 1.860 | 1.890 |
| Rpim | 0.029 | 0.352 |
| Number of reflections | 121127 | 5918 |
| <I/σ(I)> | 21 | 3.6 |
| Completeness [%] | 98.9 | 98.8 |
| Redundancy | 13.5 | 14.1 |
| CC(1/2) | 0.999 | 0.886 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | (8% (v/v) Tacsimate (pH 7.0), 17% (w/v) Polyethylene glycol 3350) |
