6IV7
The crystal structure of a SAM-dependent enzyme from aspergillus flavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.556, 62.225, 113.354 |
| Unit cell angles | 90.00, 113.06, 90.00 |
Refinement procedure
| Resolution | 41.881 - 1.937 |
| R-factor | 0.2041 |
| Rwork | 0.202 |
| R-free | 0.23770 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | PHENIX (1.14_3260) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.910 | 1.940 |
| High resolution limit [Å] | 1.937 | 1.940 |
| Rmerge | 0.092 | 0.869 |
| Rmeas | 0.109 | |
| Rpim | 0.058 | |
| Number of reflections | 72748 | 11076 |
| <I/σ(I)> | 2.15 | |
| Completeness [%] | 94.8 | 99.4 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.05M CaCl2, 0.1M Bis-Tris PH 6.5, 30% mPEG 550 |
