6IV5

Crystal structure of arabidopsis N6-mAMP deaminase MAPDA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97894
Spacegroup name	P 21 21 21
Unit cell lengths	50.904, 82.352, 86.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.853 - 1.749
R-factor	0.1893
Rwork	0.188
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a4m
RMSD bond length	0.006
RMSD bond angle	0.876
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14rc2_3191: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.710	1.840
High resolution limit [Å]	1.748	1.750
Rmerge	0.085	0.085
Number of reflections	37600	5414
<I/σ(I)>	18.1
Completeness [%]	95.6
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.8M Potassium phosphate (dibasic), 0.1M HEPES/Sodium hydroxide, 0.8M Sodium phosphate (monobasic)