6IV3

Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae with a mutation W252A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	113.461, 159.607, 162.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.106 - 2.515
R-factor	0.2341
Rwork	0.234
R-free	0.26470
RMSD bond length	0.010
RMSD bond angle	1.055
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.110	2.610
High resolution limit [Å]	2.520	2.520
Number of reflections	99774
<I/σ(I)>	12.75
Completeness [%]	98.7
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.05M zinc acetate, 6% v/v ethylene glycol, 0.1M sodium cacodylate, pH 6.0, 6.6% w/v PEG 8000