6IV0
Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae with a mutation I180A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 113.573, 159.905, 161.261 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.377 - 2.900 |
R-factor | 0.2022 |
Rwork | 0.201 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.389 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.377 | 3.004 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 65655 | |
<I/σ(I)> | 17.63 | |
Completeness [%] | 99.8 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05M zinc acetate, 6% v/v ethylene glycol, 0.1M sodium cacodylate, pH 6.0, 6.6 % w/v PEG 8000 |