6IUX
Crystal structure of a hydrolase protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.680, 52.919, 141.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.007 - 1.195 |
R-factor | 0.1597 |
Rwork | 0.159 |
R-free | 0.18280 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.959 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 1.260 |
High resolution limit [Å] | 1.195 | 1.200 |
Number of reflections | 160794 | |
<I/σ(I)> | 24.2 | |
Completeness [%] | 77.6 | |
Redundancy | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M ammonium chloride pH 6.3, 20% PEG-3350 |