6IUV
Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody 3C11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 126.780, 62.236, 127.827 |
| Unit cell angles | 90.00, 117.21, 90.00 |
Refinement procedure
| Resolution | 43.242 - 2.332 |
| R-factor | 0.2092 |
| Rwork | 0.207 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dut |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.084 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.086 | 0.395 |
| Number of reflections | 75703 | 7413 |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 99.5 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 2.0M (NH4)2SO4, phosphate-citrate pH 4.2 |
