6IUF

Crystal structure of Anti-CRISPR protein AcrVA5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 41 3 2
Unit cell lengths	143.422, 143.422, 143.422
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.807 - 2.052
R-factor	0.1541
Rwork	0.152
R-free	0.18640
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.683
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.052
Number of reflections	32118
<I/σ(I)>	52.1
Completeness [%]	99.9
Redundancy	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.1	293	0.1M NaAc, 2M (NH4)2SO4, pH 5.1