6IUB
Structure of Helicobacter pylori Soj protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2013-01-21 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.142, 93.322, 110.892 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.951 - 1.902 |
| R-factor | 0.1684 |
| Rwork | 0.166 |
| R-free | 0.21290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bek |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.372 |
| Number of reflections | 37426 | 3776 |
| <I/σ(I)> | 23.7 | 3.9 |
| Completeness [%] | 93.6 | 96.3 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 283 | Bicine (pH 9.0), Tacsimate, PEG 3350 |
