6IU7

Crystal structure of importin-alpha1 bound to the 53BP1 nuclear localization signal (wild-type)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2018-06-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	79.460, 90.150, 96.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.973 - 1.900
R-factor	0.155
Rwork	0.153
R-free	0.18510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x8n
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.1)
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.973	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.072	0.674
Rmeas	0.086	0.791
Rpim	0.045	0.408
Number of reflections	54893	3481
<I/σ(I)>	8.9
Completeness [%]	99.2	99
Redundancy	3.4	3.5
CC(1/2)	0.996	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	sodium citrate, HEPES, DTT