6ITG
a new alginate lyase (PL6) from Vibrio splendidus OU02
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2017-12-25 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 106.327, 106.327, 100.575 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.420 |
| R-factor | 0.12689 |
| Rwork | 0.125 |
| R-free | 0.16015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gkd |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.631 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.470 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Number of reflections | 216544 | |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | pH7.0, 2.4M Sodium malonate |
