6ISM
Crystal structure of intracellular B30.2 domain of BTN3A1 mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-22 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.029, 54.305, 63.027 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.140 - 1.250 |
R-factor | 0.17513 |
Rwork | 0.174 |
R-free | 0.20521 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n7u |
RMSD bond length | 0.017 |
RMSD bond angle | 1.717 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.140 | 1.290 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.050 | 0.288 |
Number of reflections | 42394 | 3880 |
<I/σ(I)> | 53.69 | |
Completeness [%] | 98.0 | |
Redundancy | 10.7 | |
CC(1/2) | 0.914 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | Magnesium chloride hexahydrate, HEPES pH 7.5, Polyethylene glycol monomethyl ether 550 |