Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 95.571, 95.571, 198.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.752 - 2.400 |
| R-factor | 0.2595 |
| Rwork | 0.258 |
| R-free | 0.29360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sp9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.017 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.213 | 1.180 |
| Number of reflections | 37032 | 1790 |
| <I/σ(I)> | 21.7 | |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 11.7 | 6.9 |
| CC(1/2) | 0.991 | 0.695 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.1M NaAc pH 5.0, 0.1M NaCl, 35% PEG 400 |
