6ISC

complex structure of mCD226-ecto and hCD155-D1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97923
Spacegroup name	C 2 2 21
Unit cell lengths	50.220, 78.303, 184.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.273 - 2.200
R-factor	0.2347
Rwork	0.233
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6isa 3EOW
RMSD bond length	0.002
RMSD bond angle	0.489
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.099
Rpim	0.036	0.378
Number of reflections	18895	1772
<I/σ(I)>	23.456
Completeness [%]	99.5	96.2
Redundancy	8.6	6
CC(1/2)	0.999	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Tris (pH 8.0), 0.15 M ammonium sulfate, and 15% (w/v) PEG4000