6ISB
crystal structure of human CD226
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.879, 118.492, 123.171 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.696 - 2.500 |
R-factor | 0.2555 |
Rwork | 0.255 |
R-free | 0.26730 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.872 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.077 | 0.573 |
Rpim | 0.030 | 0.213 |
Number of reflections | 30085 | 2955 |
<I/σ(I)> | 24.645 | 4.311 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.9 | 8.2 |
CC(1/2) | 0.994 | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 277 | 0.1 M Tris (pH 7.5) and 0.8 M Na/K hydrogen phosphate |