6IS2
Crystal Structure of Staphylococcus aureus response regulator ArlR receiver domain in complex with Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.589, 45.795, 61.847 |
| Unit cell angles | 99.72, 102.26, 86.50 |
Refinement procedure
| Resolution | 31.700 - 1.591 |
| R-factor | 0.1564 |
| Rwork | 0.155 |
| R-free | 0.18830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.038 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.700 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Number of reflections | 58295 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 94.3 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Magnesium chloride hexahydrate, 0.1M HEPES pH 7.5 and 25%(w/v) Polyethylene glycol 3,350 |
