6IRX
Crystal structure of the zebrafish cap-specific adenosine methyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.545, 84.132, 93.084 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.033 - 2.000 |
R-factor | 0.1923 |
Rwork | 0.191 |
R-free | 0.21540 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.2) |
Phasing software | SHELXD |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.033 | 47.030 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.135 | 0.085 | 1.054 |
Rmeas | 0.141 | 0.089 | 1.098 |
Rpim | 0.040 | 0.026 | 0.303 |
Number of reflections | 38694 | 511 | 2822 |
<I/σ(I)> | 10.1 | ||
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 12.4 | 11 | 12.9 |
CC(1/2) | 0.996 | 0.990 | 0.913 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 11% PEG 3350, 0.1M potassium thiocyanate, 0.1M bis-Tris propane |