6IRQ

Complexed crystal structure of PaSSB with ssDNA dT25 at 1.91 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	298
Detector technology	CCD
Collection date	2017-10-06
Detector	ADSC QUANTUM 210
Wavelength(s)	0.975
Spacegroup name	P 31
Unit cell lengths	60.488, 60.488, 131.315
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.472 - 1.910
R-factor	0.2147
Rwork	0.213
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eyg
RMSD bond length	0.008
RMSD bond angle	0.874
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.980
High resolution limit [Å]	1.910	1.910
Rmerge	0.047	0.401
Number of reflections	40690
<I/σ(I)>	24.23	2.6
Completeness [%]	97.4	97.1
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	30% PEG400, 100mM MES Sodium salt pH6.5, 100mM Magnesium Chloride