6IRP
Crystal structure of HigA from Shigella flexneri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 80.366, 80.366, 90.385 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.799 - 1.954 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.23060 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.835 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.10.1_2155) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 24365 | |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% PEG3350, 0.1M HEPES pH 7.5 , 0.2 M lithium sulfate monohydrate |
