6IRB
C-terminal coiled coil domain of Drosophila phospholipase C beta NORPA, selenomethionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 |
Unit cell lengths | 36.958, 37.591, 140.130 |
Unit cell angles | 90.72, 89.86, 119.44 |
Refinement procedure
Resolution | 14.904 - 2.661 |
R-factor | 0.2276 |
Rwork | 0.225 |
R-free | 0.27980 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
Rmerge | 0.091 | 0.065 | 0.827 |
Rmeas | 0.108 | 0.077 | 0.984 |
Rpim | 0.058 | 0.042 | 0.530 |
Total number of observations | 59839 | ||
Number of reflections | 17843 | 882 | 834 |
<I/σ(I)> | 10.6 | ||
Completeness [%] | 96.6 | 95.4 | 95.4 |
Redundancy | 3.4 | 3.4 | 3.3 |
CC(1/2) | 0.997 | 0.785 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 7.2 | 289 | 64% 2-Methyl-2,4-pentanediol (MPD), 100mM HEPES, 8% Pentaerythritol ethoxylate (3/4 EO/OH) |