6IR7
Green fluorescent protein variant GFPuv with the modification to 6-hydroxynorleucine at the C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.295, 51.152, 48.506 |
| Unit cell angles | 90.00, 100.79, 90.00 |
Refinement procedure
| Resolution | 47.649 - 1.277 |
| R-factor | 0.1758 |
| Rwork | 0.175 |
| R-free | 0.19510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b9c |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.140 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Number of reflections | 56911 | 2566 |
| <I/σ(I)> | 27.8 | 7.2 |
| Completeness [%] | 96.3 | 87.4 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammonium Sulfate, 0.1 M MES pH 6.5, 30% PEGMME5000 |
