6IR5
P domain of GII.3-TV24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 121.974, 121.974, 216.568 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.486 - 2.600 |
| R-factor | 0.2012 |
| Rwork | 0.199 |
| R-free | 0.23540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pum |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.590 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.171 | 0.668 |
| Number of reflections | 51158 | 5000 |
| <I/σ(I)> | 10.7 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 9.6% (w/v) polyethylene glycol 3350, 160mM ammonium citrate tribasic (pH 7.0), 20mM tri-sodium citrate dehydrate (pH 5.6), 500mM 1,6-hexanediol, 30mM glycl-glycl-glycin |
