6IR4

Crystal structure of BioU from Synechocystis sp.PCC6803 (apo form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	71.273, 71.273, 98.737
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.720 - 2.000
R-factor	0.21005
Rwork	0.207
R-free	0.27408
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.413
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.725	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.117	0.662
Rpim	0.041	0.218
Number of reflections	20146
<I/σ(I)>	27.1
Completeness [%]	100.0
Redundancy	9.7
CC(1/2)	0.979	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	20% (w/v) PEG 3350, 0.2M ammonium formate