6IR4
Crystal structure of BioU from Synechocystis sp.PCC6803 (apo form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 71.273, 71.273, 98.737 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 61.720 - 2.000 |
R-factor | 0.21005 |
Rwork | 0.207 |
R-free | 0.27408 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.413 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.725 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.117 | 0.662 |
Rpim | 0.041 | 0.218 |
Number of reflections | 20146 | |
<I/σ(I)> | 27.1 | |
Completeness [%] | 100.0 | |
Redundancy | 9.7 | |
CC(1/2) | 0.979 | 0.857 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% (w/v) PEG 3350, 0.2M ammonium formate |