6IR2
Crystal structure of red fluorescent protein mCherry complexed with the nanobody LaM2 at 1.4 Angstron resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97930 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.817, 61.046, 109.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.396 - 1.393 |
R-factor | 0.1961 |
Rwork | 0.195 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h5q 3k1k |
RMSD bond length | 0.006 |
RMSD bond angle | 1.026 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.450 |
High resolution limit [Å] | 1.393 | 3.020 | 1.400 |
Rmerge | 0.139 | 0.074 | 1.504 |
Rmeas | 0.145 | 0.078 | 1.599 |
Rpim | 0.043 | 0.023 | 0.532 |
Number of reflections | 65784 | 6678 | 6407 |
<I/σ(I)> | 7.5 | ||
Completeness [%] | 97.9 | 94.4 | 97 |
Redundancy | 11.2 | 10.7 | 8.2 |
CC(1/2) | 0.997 | 0.662 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.2M Magnesium chloride hexahydrate, 0.1M Tris pH 8.5, 25%(w/v) Polyethylene glycol 3,350. |