6IQV
Crystal Structure of Cell Surface Glyceraldehyde-3-Phosphate Dehydrogenase Complexed with Hg2+ from Lactobacillus plantarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-31 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0085 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 126.043, 172.949, 149.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 101.860 - 2.130 |
| R-factor | 0.20464 |
| Rwork | 0.202 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j9g |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.970 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.860 | 2.170 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.090 | 0.521 |
| Number of reflections | 90883 | |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 9.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 25% (w/v) PEG 1500, 0.1M MIB buffer (pH 5.0) |
