6IQO
Se-Met L45M Programmed Cell Death 5 protein from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.085, 40.860, 77.819 |
| Unit cell angles | 90.00, 129.73, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.110 |
| R-factor | 0.228 |
| Rwork | 0.225 |
| R-free | 0.28385 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.574 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.210 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.055 | 0.377 |
| Rmeas | 0.059 | 0.414 |
| Rpim | 0.023 | 0.168 |
| Number of reflections | 15111 | 1410 |
| <I/σ(I)> | 30.13 | 4.09 |
| Completeness [%] | 98.6 | 92.3 |
| Redundancy | 6.8 | 5.6 |
| CC(1/2) | 0.928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 0.2 M NaCl, 25 % PEG3350, 0.1 M Bis-Tris pH 5.5 |
