6IQK

crystal structure of Arabidopsis thaliana Profilin 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X17B1
Synchrotron site	NSLS
Beamline	X17B1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-12-03
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	135.900, 153.370, 196.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.240 - 3.600
R-factor	0.3128
Rwork	0.309
R-free	0.34650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.549
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.240	3.729
High resolution limit [Å]	3.600	3.600
Number of reflections	48279	48279
<I/σ(I)>	6
Completeness [%]	99.8
Redundancy	15
CC(1/2)	0.991	0.275

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.1M Tris HCl (pH 7.8), 0.9M sodium citrate