6IQH
X-ray crystal structure of covalent-bonded complex of Fc and peptide
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.082, 66.988, 78.336 |
| Unit cell angles | 90.00, 106.41, 90.00 |
Refinement procedure
| Resolution | 44.100 - 2.999 |
| R-factor | 0.2269 |
| Rwork | 0.223 |
| R-free | 0.29270 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1dn2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.633 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.100 | 3.110 |
| High resolution limit [Å] | 2.999 | 3.000 |
| Rmerge | 0.159 | 0.417 |
| Number of reflections | 12253 | 1158 |
| <I/σ(I)> | 8.4 | 1.6 |
| Completeness [%] | 98.4 | 92.9 |
| Redundancy | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 293 | 0.1 M CHES pH 10.0, 0.3M NaCl, 20% (w/v) PEG 8000 |
