6IPY
His-tagged Fyn SH3 domain R96I mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-05 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 41 |
| Unit cell lengths | 41.520, 41.520, 32.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.520 - 1.343 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.20130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h0h |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.520 | 1.391 |
| High resolution limit [Å] | 1.343 | 1.343 |
| Rmerge | 0.073 | 1.374 |
| Rmeas | 0.076 | 1.479 |
| Rpim | 0.021 | 0.523 |
| Number of reflections | 12432 | 1125 |
| <I/σ(I)> | 20.23 | 0.76 |
| Completeness [%] | 98.6 | 87.67 |
| Redundancy | 12.5 | 7.3 |
| CC(1/2) | 0.999 | 0.555 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 295 | 0.1M Citric acid pH 3.5 and 25%(w/v) Polyethylene glycol 3,350 |
