6IPV
Crystal structure of CqsB2 from Streptomyces exfoliatus 2419-SVT2 (apo form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-25 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 127.687, 127.687, 125.724 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 125.720 - 2.200 |
R-factor | 0.18369 |
Rwork | 0.181 |
R-free | 0.22913 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.368 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 125.720 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.128 | 0.278 |
Rpim | 0.054 | 0.266 |
Number of reflections | 53154 | 2605 |
<I/σ(I)> | 21.9 | |
Completeness [%] | 100.0 | |
Redundancy | 14.7 | |
CC(1/2) | 0.991 | 0.879 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl (pH8.5), 30% (w/v) PEG 4000, 0.2M LiSO4 |