6IP7
Structure of human telomeric DNA with 5-Selenophene-modified deoxyuridine at residue 11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 6 |
Unit cell lengths | 56.483, 56.483, 42.415 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.916 - 1.550 |
R-factor | 0.1907 |
Rwork | 0.189 |
R-free | 0.21290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kf1 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.424 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.058 | 0.898 |
Number of reflections | 22608 | 563 |
<I/σ(I)> | 23.6 | |
Completeness [%] | 100.0 | |
Redundancy | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.05M potassium cacodylate (pH7.2), 0.625M ammonium acetate, 0.2M KCl, 15% PEG400 |