6IP3
Structure of human telomeric DNA at 1.4 Angstroms resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97949 |
Spacegroup name | P 6 |
Unit cell lengths | 56.839, 56.839, 42.411 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.420 - 1.400 |
R-factor | 0.1523 |
Rwork | 0.151 |
R-free | 0.17270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kf1 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.100 | 0.880 |
Number of reflections | 15493 | 777 |
<I/σ(I)> | 16.4 | 3.5 |
Completeness [%] | 100.0 | |
Redundancy | 16.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.05M sodium cacodylate (pH = 7.2), 0.4M ammonium sulfate, 0.05M KCl, 0.01M CaCl2, 15% PEG400 |