6IOW
Crystal structure of Porphyromonas gingivalis phosphotransacetylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2018-02-13 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.355, 69.066, 201.947 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.440 - 1.700 |
| R-factor | 0.21081 |
| Rwork | 0.209 |
| R-free | 0.24435 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2af4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.035 |
| Data reduction software | DIALS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.320 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.065 | 0.126 |
| Number of reflections | 67506 | 2615 |
| <I/σ(I)> | 23.28 | 5.7 |
| Completeness [%] | 97.2 | 76.4 |
| Redundancy | 12.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (w/v) polyethylene glycol (PEG) 3350 and 0.2 M ammonium tartrate dibasic |
