6IOV
The ligand binding domain of Mlp37 with arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2015-10-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.593, 100.482, 106.806 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.157 - 2.351 |
| R-factor | 0.1903 |
| Rwork | 0.185 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ave |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.075 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.200 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.096 | 0.429 |
| Number of reflections | 22612 | 2141 |
| <I/σ(I)> | 14.2 | 4.2 |
| Completeness [%] | 98.8 | 97.3 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES pH 7.5, 2.0M ammonium sulfate, 1.2% (v/v) PEG 400 |
