6IOS
The ligand binding domain of Mlp24 with proline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-05-22 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 59.110, 111.200, 113.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.600 - 2.350 |
| R-factor | 0.2164 |
| Rwork | 0.214 |
| R-free | 0.25490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.180 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.600 | 2.440 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.109 | 0.423 |
| Number of reflections | 15952 | 1638 |
| <I/σ(I)> | 14.5 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris pH 8.5, 50% (v/v) PEG 400, 0.1M calcium acetate |
