6IOS
The ligand binding domain of Mlp24 with proline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2014-05-22 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 1.00 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 59.110, 111.200, 113.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.600 - 2.350 |
R-factor | 0.2164 |
Rwork | 0.214 |
R-free | 0.25490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.180 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.600 | 2.440 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.109 | 0.423 |
Number of reflections | 15952 | 1638 |
<I/σ(I)> | 14.5 | 4.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris pH 8.5, 50% (v/v) PEG 400, 0.1M calcium acetate |