6IOP
The ligand binding domain of Mlp24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-07-05 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.100, 88.390, 64.530 |
| Unit cell angles | 90.00, 105.00, 90.00 |
Refinement procedure
| Resolution | 36.052 - 2.300 |
| R-factor | 0.2119 |
| Rwork | 0.209 |
| R-free | 0.26380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c8c |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.213 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.900 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.107 | 0.359 |
| Number of reflections | 25138 | 2477 |
| <I/σ(I)> | 7.2 | 3.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES pH 6.0, 42.5% (v/v) PEG 400, 0.1M Calcium acetate and 3% (v/v) DMSO |
