6IOM
Crystal structure of human C4.4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9196 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.055, 120.156, 169.431 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.309 - 2.594 |
| R-factor | 0.2007 |
| Rwork | 0.198 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ion |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.413 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.309 | 2.690 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.094 | 0.690 |
| Number of reflections | 18403 | 1763 |
| <I/σ(I)> | 37.58 | |
| Completeness [%] | 99.5 | |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.6 | 293 | 22.5%(w/v) polyethylene glycol 4000, 0.1M citric acid in pH 3.6 |
